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Aug 21, 2023

Effects of acidification on nitrification and associated nitrous oxide emission in estuarine and coastal waters

Nature Communications volume 14, Article number: 1380 (2023) Cite this article 5487 Accesses 25 Altmetric Metrics details In the context of an increasing atmospheric carbon dioxide (CO2) level,

Nature Communications volume 14, Article number: 1380 (2023) Cite this article

5487 Accesses

25 Altmetric

Metrics details

In the context of an increasing atmospheric carbon dioxide (CO2) level, acidification of estuarine and coastal waters is greatly exacerbated by land-derived nutrient inputs, coastal upwelling, and complex biogeochemical processes. A deeper understanding of how nitrifiers respond to intensifying acidification is thus crucial to predict the response of estuarine and coastal ecosystems and their contribution to global climate change. Here, we show that acidification can significantly decrease nitrification rate but stimulate generation of byproduct nitrous oxide (N2O) in estuarine and coastal waters. By varying CO2 concentration and pH independently, an expected beneficial effect of elevated CO2 on activity of nitrifiers (“CO2-fertilization” effect) is excluded under acidification. Metatranscriptome data further demonstrate that nitrifiers could significantly up-regulate gene expressions associated with intracellular pH homeostasis to cope with acidification stress. This study highlights the molecular underpinnings of acidification effects on nitrification and associated greenhouse gas N2O emission, and helps predict the response and evolution of estuarine and coastal ecosystems under climate change and human activities.

Carbon dioxide (CO2) in the atmosphere has been increasing due to intensive human activities such as combustion of fossil fuels, cement production, deforestation, and other land-use changes1. Globally, the average atmospheric concentration of CO2 has now reached 413.2 ppm and is expected to exceed 800 ppm by the end of the 21st century2,3. Approximately 40% of the emitted CO2 during the industrial era has been absorbed by the oceans4, consequently causing a reduction of about 0.1 pH unit in surface seawater5,6. A further decline of 0.2–0.3 pH units is estimated at the end of this century, with severe consequences expected for sensitive organisms and ecosystems6,7,8.

Estuarine and coastal ecosystems are dynamic regions under the interaction of rivers, land, and oceans9, which can provide vital ecosystem services for human well-being10. In the context of an increasing atmospheric CO2 level, estuarine and coastal waters, however, suffer from more acute acidification than open oceans, under the synergistic effects of land-derived nutrient inputs, coastal upwelling, and complex biogeochemical processes (Supplementary Fig. 1)11,12. One of the greatest threats to estuarine and coastal ecosystems worldwide is the excess input of watershed anthropogenic nutrients10. The eutrophication-induced phytoplankton production can result in high respiration rate in bottom waters where the algal-derived matter settles, which may cause strong CO2 production13. Acidification in estuarine and coastal waters can thus be greatly intensified by episodic intrusion of high-CO2 upwelled water11,13,14, which may detrimentally affect biological processes and functioning of estuarine and coastal ecosystems15,16,17,18,19,20,21.

Nitrification is a critical process for the balance of reduced and oxidized nitrogen pools, linking mineralization to nitrogen removal pathways of denitrification and anaerobic ammonium oxidation22. It thus plays a crucial role in the global nitrogen cycle, especially in eutrophic aquatic ecosystems. Due to the slow growth of nitrifiers and their high sensitivity to environmental perturbations23, nitrification is anticipated to be disturbed by aquatic acidification. One complication in the response of nitrifiers to acidification is that the increase of partial pressure of CO2 (pCO2) and the decrease of pH may have opposing effects. Higher pCO2 condition is expected to benefit nitrification, as an increased carbon source may promote the growth of chemoautotrophic nitrifiers (CO2-fertilization)24,25,26,27. In contrast, the concomitant decrease in pH can shift the equilibrium between ammonia (NH3) and ammonium (NH4+) toward a lower concentration of substrate NH3 available for ammonia-oxidizers and thereby inhibit nitrification25,28,29. The response of nitrifiers thus depends strongly on the balance of these potential positive and negative effects. However, little is known concerning the effects of projected levels of aquatic acidification on the metabolisms of nitrifiers and underlying mechanisms.

Nitrification is also an important pathway for production of greenhouse gas nitrous oxide (N2O)30,31,32,33, which has >300-fold stronger radiative forcing per mole than CO2 and can react with ozone in the stratosphere34. N2O can be enzymatically produced by ammonia-oxidizing bacteria (AOB) via conversion of hydroxylamine (NH2OH) to N2O35,36, or via nitrifier denitrification [a sequential reduction of nitrite (NO2−) to nitric oxide (NO) and N2O]35. Recently, the biotic conversion of NH2OH to N2O by AOB through the cytochrome P460 was also characterized36. In contrast, N2O emission associated with NH3 oxidation by ammonia-oxidizing archaea (AOA) is believed to result mainly from abiotic reactions between NH2OH and NO2− or NO32,37,38. Previous studies suggested that AOA produce lower yields of N2O than AOB during aerobic NH3 oxidation37,39. In addition, it has been documented that complete ammonia oxidizers (comammox) exhibit a lower N2O yield than AOB, as N2O originates rather from the abiotic conversion of NH2OH by comammox bacteria40,41. However, it is not yet clear how nitrifier N2O production will respond to aquatic acidification.

Here we examine how aquatic acidification affects nitrification rate and associated N2O emission in the Yangtze Estuary and adjacent coastal waters. Manipulation experiments are also conducted to decouple the individual effects of elevated pCO2 and reduced pH. Metatranscriptomes are further analyzed to elucidate the metabolic response of nitrifying microbes by tracking the expression of acidification responsive genes. This research provides insights into the mechanistic interactions between acidification and nitrification, and helps predict the future ecological evolution of estuarine and coastal ecosystems.

Different acidification levels (pH reduced by 0.10–1.05) were achieved via bubbling water samples collected from six representative sites (Yz1–Yz6) along the Yangtze Estuary and adjacent coastal waters with different air:CO2 gas mixtures (Fig. 1, Supplementary Tables 1 and 2). Nitrification rates at these sites showed an identical response to acidification: all decreased remarkably when pH was reduced regardless of a potential beneficial effect of high CO2 (Supplementary Fig. 2). A decrease in nitrification rates (5.8–18.1%) was detected under pH reduction even from 7.92–8.15 to 7.80–8.04 (pCO2 increased by 122–172 μatm) (P < 0.05). Nitrification rates decreased by ~11.1–34.1% when the pCO2 was doubled (P < 0.05). The decrease of nitrification rates was strongly correlated with the reduction of water pH (P < 0.05), based on the constructed acidification–response curves (Fig. 2a). Nitrification rates would decrease by ~7.7–25.0% under an average reduction of about 0.21 pH units which has been observed in estuarine and coastal waters across the world over the past several decades (Supplementary Fig. 1). This inhibition effect of acidification on nitrification rate is consistent with what was previously observed in the open oceans28,42. Whilst nitrification rate was reported to increase along a decreasing natural gradient of pH in Narragansett Bay43, it was likely due to a combination of biogeochemical conditions rather than the effect of acidification alone.

Stations are marked by red stars.

a Nitrification rates. Data show the percentage changes of nitrification rates in the acidified treatments compared to the ambient control. For all the lines, P < 0.05. b N2O production rates. Data show the percentage changes of N2O production rates in the acidified treatments compared to the ambient control. For all the lines, P < 0.05. Error bars represent SD (n = 3 biologically independent samples). ΔpH corresponds to the decrease between water pH before and after acidification. The fitting curve was obtained by polynomial fitting method. Equations and P values for the fitted curves are given in Supplementary Table 3. Source data are provided as a source data file.

The inhibition of nitrification rate by acidification tended to be lower in the upper estuary waters where AOB were the dominant ammonia oxidizers than that in the adjacent coastal regions where the ammonia-oxidizing communities were dominated by AOA (Fig. 2a and Supplementary Fig. 3). In addition to the heterogeneity of nitrifying microbial communities, this variability in the influence of acidification on nitrification rate may stem from multiple biogeochemical factors43. Especially, the relatively higher NH4+ concentrations in the upper estuaries may mitigate the inhibiting effects on ammonia oxidation caused by acidification (Supplementary Table 1). Consistently, the measured inhibition of nitrification rate by acidification in the estuarine and coastal waters was generally lower than that in the oligotrophic seas where nitrification rates were reported to decline by 3–44% in response to a decrease of 0.1 pH unit28,42. Indeed, NH4+ concentration was negatively correlated with the inhibition effect of acidification on nitrification rate in different habitats ranging from estuary to open ocean (P < 0.05)28,42,44 (Supplementary Fig. 4). Nevertheless, considering the faster and continuous aggravation of acidification in estuarine and coastal waters due to the synergistic effects of human activity-induced eutrophication and elevated atmospheric CO2 level11, its disturbance on nitrification rates could cause profound consequences on estuarine and coastal ecosystem processes.

Based on the response of nitrification rates, ref. 28 speculated that the decrease in nitrification rates as a result of ocean acidification could greatly reduce N2O production in the open ocean. The impact of aquatic acidification on the N2O production, however, may be decoupled from its impact on the nitrification rates45. For example, ref. 42 recently reported that when seawater pH in the western North Pacific was reduced, the N2O production increased significantly while nitrification rates remained stable or even decreased. However, in their work, the acidification was manipulated by adding strong acid (HCl). Although the addition of HCl can elevate pCO2 and reduce pH, it also alters alkalinity and results in different carbonate parameters compared with those expected in the future, i.e., dissolved inorganic carbon (DIC) increases under natural aquatic acidification rather than remains unchanged46. Moreover, nitrifying communities in diverse aquatic habitats may respond differently to acidification, as the mechanisms for N2O production differ among different nitrifying organisms32,38,40. Therefore, the response of N2O production during nitrification to aquatic acidification in estuarine and coastal waters needs to be evaluated.

Through aerating with sterile air at different CO2 levels47, which can best mimic the ongoing aquatic acidification, we found that the production rates of N2O at all sampling sites were enhanced significantly by acidification (Supplementary Fig. 5). Even under a pH decline of ~0.1 unit (7.92–8.15 to 7.80–8.04), a significant promotion of N2O production (8.4–23.1%) was observed at the end of incubation (P < 0.05). Acidification–response curves were constructed between the decline in pH and N2O emission, showing a significant increase of N2O production rates along with the increase of acidification levels (P < 0.05) (Fig. 2b). Under an average reduction of about 0.21 pH units detected in estuarine and coastal regions worldwide, the rates of N2O production during nitrification might increase by ~9.5–27.5% (Fig. 2b). These findings support our hypothesis that, similar to other environmental perturbations such as low oxygen and toxicant exposures23,42,48, acidification can increase N2O production in estuarine and coastal waters, regardless of whether AOB or AOA dominated. Therefore, although the mechanisms for N2O production differ among different nitrifying organisms32,38,40, their N2O production rate might increase under pH reduction. The increased N2O production under acidified conditions in estuarine and coastal waters is consistent with those in the western North Pacific42. However, ref. 44 documented inhibition of N2O production by ocean acidification in cold temperate and polar seawaters. Assuming that nitrification is the main N2O production pathway in their study, the response of the N2O production to acidification would be different in polar seas. Although heterotrophic denitrifiers may also contribute to the production of N2O, their contribution may be insignificant, as the natural isotopic signatures of N2O showed that the pathway of NO2− reduction (including nitrifier denitrification and heterotrophic denitrification) contributed only 0–13.3% of the released N2O (Supplementary Table 4). Moreover, the samples from all study sites were well oxygenated [dissolved oxygen (DO): 8.30–9.86 mg L−1; Supplementary Table 1] and remained at high DO levels during the incubation (Supplementary Table 2), which was unlikely to occur for heterotrophic denitrification. Previous studies reported that when the DO concentration is more than 0.06 mg L−1, the N2O production by heterotrophic denitrification is completely inhibited49. Therefore, the contribution of denitrifying bacteria to the production of N2O should be negligible. This study demonstrates that N2O production during nitrification of both AOB-dominated and AOA-dominated nitrifiers can be stimulated by acidification, which is an important step for evaluating the impact of ongoing acidification on N2O emission in the complex and dynamic estuarine and coastal ecosystems. Should our results be representative and that nitrifiers contribute to half of global estuarine and coastal N2O emissions50,51 (Supplementary Fig. 6 and Supplementary Table 5), nitrification-derived N2O emission in these ecosystems would increase by 0.05–0.15 Tg N2O-N yr−1 in response to an average decrease of 0.21 pH units (Supplementary Text 1), accounting for 0.7–2.2% of the total anthropogenic N2O emissions globally (6.9 Tg N2O-N yr−1)52.

Our results showed that acidification can inhibit nitrification rates but enhance N2O emissions in estuarine and coastal waters (Fig. 2). However, the increase in pCO2 and decrease in pH may have opposing effects on nitrifiers. To distinguish the individual effects of elevated pCO2 and reduced pH, a series of open, continuous-flow microcosm systems were constructed. The carbonate chemistry was manipulated by steadily bubbling collected water samples from site Yz3 with CO2 adjusted air (400 μatm and 800 μatm) while adjusting pH (7.8 and 8.1) with sterile acid or base solution according to a real-time pH detector (Supplementary Fig. 7). Equilibrium states were achieved in four scenarios [low pCO2 (400 μatm) and high pH (8.1); low pCO2 (400 μatm) and low pH (7.8); high pCO2 (800 μatm) and low pH (7.8); high pCO2 (800 μatm) and high pH (8.1)] and remained stable during the incubation period (Supplementary Table 6). Results showed that nitrification rates significantly decreased at low pH, independent of pCO2 level (Fig. 3a), demonstrating the negative effects of reduced pH on nitrification rates. However, the effect of elevated pCO2, which is expected to benefit nitrification, seems to be pH-dependent (Fig. 3a). When pH was maintained at the ambient 8.1, an obvious “CO2-fertilization” effect was observed as nitrification rates increased at high pCO2 (Fig. 3a). In contrast, under acidified conditions (pH 7.8), elevated pCO2 caused an unexpected decrease in nitrification rates (Fig. 3a). This pattern suggests that, when the metabolic processes of the related nitrifiers are affected by reduced pH, increased pCO2 becomes an additive stressor. These results are in contrast to the observation from N2-fixing cyanobacteria, which can benefit from high pCO2 under reduced pH conditions53.

a Nitrification rates. b N2O production rates during nitrification. Four scenarios were constructed: the ambient control (400 μatm/pH 8.1, blue solid bars), the acidified group (800 μatm/pH 7.8, red solid bars), reduction of pH only (400 μatm/pH 7.8, red open bars), and increase of pCO2 only (800 μatm/pH 8.1, blue open bars). Different lowercase letters (a, b, c and d) above the columns indicate significant differences among the four scenarios (P < 0.05). Error bars represent SD (n = 3 biologically independent samples), and dots are corresponding data points of the replicates. Significant differences were determined via one-way analysis of variance (ANOVA). Source data are provided as a source data file.

N2O production during nitrification was promoted under acidified conditions (Fig. 2b), by both the reduced pH and the elevated pCO2 (Fig. 3b). The promotion of N2O production under high pCO2 while maintaining the ambient pH was unexpected (Fig. 3b) as this condition was beneficial for nitrifiers (CO2-fertilization; Fig. 3a). It is possible that the production of by-product N2O could increase along with the increase of nitrification rates. However, this possibility cannot fully explain the enhanced N2O emission under the condition of sole pCO2 elevation (pCO2 800 μatm/pH 8.1), as the promotion of N2O production rate was significantly higher than the nitrification rate (Fig. 3). Under natural acidified conditions (an elevation of pCO2 concomitant with pH reduction), the strongest promotion of N2O emission may be expected, when the effects of pH reduction and pCO2 elevation are combined (Fig. 3b). This is the first attempt to decouple the individual effects of elevated pCO2 and reduced pH on nitrification rate and associated N2O emission in acidified aquatic environments, providing insights into the underlying mechanism of aquatic acidification affecting nitrifiers. Their individual effects were successfully distinguished based on our constructed continuous-airflow and automatic pH incubation systems. It was previously speculated that increasing levels of pCO2 may cause positive effect on the activity of chemoautotrophic nitrifiers54. In contrast, under acidified conditions, elevated pCO2 may further inhibit nitrification rate and promote the undesirable by-product N2O emission. Therefore, the negative effects of aquatic acidification on microbial nitrogen transformations and their feedback to global climate change are probably more intensive than previously thought (Supplementary Fig. 8).

As an important molecular response to acidification stress, nitrifiers may adjust gene expressions by intracellular signaling networks55, which can be further reflected by reduced nitrification rate and enhanced N2O production. However, the transcriptional response of nitrifying communities to aquatic acidification remains unknown. Metatranscriptome sequencing can be used to interrogate the differential expression of genes involved in the physiological metabolism of nitrifying communities under acidified conditions23. However, previous attempts to acquire metatranscriptomes based on the short-term acidification experiments failed, because there was not enough mRNA with good integrity and purity, which might be due to the extreme instability of mRNA and the complexity of environmental samples23. More importantly, nitrifiers account for only a small fraction of the complex microbial communities in estuarine and coastal waters (<5%; Supplementary Fig. 9), thus it is difficult to extract sufficient nitrifying transcripts to fully reveal the physiological metabolism of nitrifiers. Therefore, a series of continuous-flow environmental simulators with water samples from site Yz3 were set up to mimic long-term acidification and to enrich nitrifiers. The pH and pCO2 in the ambient controls were maintained at about 8.1 and 400 μatm, respectively, whereas they were stabilized at about 7.8 and 800 μatm, respectively, in the acidified treatments. After ~25 days, the continuously operated simulators exhibited stable nitrification reactions (Supplementary Fig. 10) and dominance of nitrifying communities (accounting for 44.6% and 45.5% in the ambient controls and acidified treatments, respectively) (Supplementary Figs. 11,12). The in-situ nitrifying bacteria were simultaneously enriched, and the original members in the environment (Nitrosomonas and Nitrospira) remained dominant. However, the cell numbers of AOA were not greatly increased in the nitrifying enrichments, and thus AOA cultivation was further conducted with streptomycin to inhibit nitrifying bacteria. After 50 days of incubation, the relative abundance of AOA increased from 1.1% to 11.2%, whereas nitrifying bacteria were undetectable (Supplementary Fig. 13). For these nitrifying enrichment cultures, significant reduced nitrification rates and stimulated N2O emissions were observed in the acidified treatments (Supplementary Figs. 14, 15), a pattern consistent with that of the field water samples. Although the enrichment manipulation changes the original environmental microbial communities, the nitrifying enrichments are representatives to investigate the transcriptional response of nitrifiers to acidification in complex estuarine and coastal waters.

According to the metatranscriptomic analyses (Supplementary Table 7), CO2-induced acidification can significantly affect the physiological metabolisms of nitrifiers at transcriptional level, as those genes involved in nitrogen transformations, cytosolic pH homeostasis, energy generation, and CO2 fixation were all greatly regulated by acidification (Figs. 4a–c, 5a–c). Expression of the potentially active subunit of the ammonia monooxygenase gene (amoA) of AOB was down-regulated by 66% for the acidified treatments (Fig. 4a). Real-time quantitative polymerase chain reaction (qPCR) also demonstrated that the expression of bacterial amoA gene was significantly down-regulated under acidified conditions (P < 0.01; Supplementary Table 9). Consistently, expressions of bacterial amoB (ammonia monooxygenase subunit B, also suggested as a catalytic subunit56) and amoC (ammonia monooxygenase subunit C) decreased by 68 and 69%, respectively, after acidification (Fig. 4c). In addition, expression of hydroxylamine dehydrogenase gene (hao) was down-regulated by 61% (P < 0.01), while the expressions of nitrite oxidoreductase genes nxrA and nxrB were reduced by 76 and 61% (P < 0.01), respectively, under acidified conditions (Fig. 4c and Supplementary Table 9). Based on the transcriptional data of AOA, expression of archaeal amoA was also down-regulated (24%) under acidified conditions (P < 0.05) (Fig. 5a and Supplementary Table 9). Additionally, transcripts of archaeal amoC were down-regulated by 43%, while the expression of archaeal amoB remained unchanged under acidification (Fig. 5c). Overall, the functional gene transcripts involved in the stepwise oxidation of NH3 to nitrate (NO3−) were generally down-regulated under acidified conditions, consistent with the reduction of nitrification rates (Fig. 2a).

a Schematic model depicting the effects of acidification on the expression of genes involved in the stepwise oxidation of NH3 (NH3 → NH2OH → NO → NO2− → NO3−), N2O production, energy generation, and cytosolic pH homeostasis of nitrifying bacteria. The membrane is broken by dotted line, as nitrite oxidation does not often occur in the same organism with ammonia oxidation, with the exception of recently discovered comammox Nitrospira. Fold change (FC) in relative gene expression was calculated by comparing the acidified samples to the ambient control. The roman numbers refer to the enzyme complex I (NADH dehydrogenase), complex III (cytochrome bc1 complex), complex IV (cytochrome c oxidase), and complex V (ATP synthase) in the respiratory chain. Dotted blue arrows show the movement of electrons. E, unknown enzyme. b Effects of acidification on the expression of genes involved in CO2 fixation through Calvin and reductive tricarboxylic acid (rTCA) cycles. Colors at the center of the protein pictograms indicate the extent of up- or down-regulation of gene transcripts encoding these proteins. Colors at the periphery of the protein pictograms correspond to bar colors in (c). c FC of transcripts encoding proteins showed in (a) and (b). Definitions of the abbreviations are shown in Supplementary Table 8. Source data are provided as a source data file.

a Schematic model depicting the effects of acidification on the expression of genes involved in NH3 oxidation, N2O production, energy generation, and cytosolic pH homeostasis of AOA. Fold change (FC) in relative gene expression was calculated by comparing the acidified samples to the ambient control. The roman numbers refer to the enzyme complex I (NADH dehydrogenase), complex III (b-pcy), complex IV (pcy-aa3), and complex V (ATP synthase) in the respiratory chain. Dotted blue arrows show the movement of electrons. b Effects of acidification on the expression of genes involved in 3-hydroxypropionate/4-hydroxybutyrate cycle. Colors at the center of the protein pictograms indicate the extent of up- or down-regulation of gene transcripts encoding these proteins. Colors at the periphery of the protein pictograms correspond to bar colors in (c). c FC of transcripts encoding proteins showed in (a) and (b). Definitions of the abbreviations are shown in Supplementary Table 8. Source data are provided as a source data file.

In contrast, transcripts of genes encoding nitrifying bacterial nitric oxide reductase (N2O-forming, nor) were up-regulated by acidification (Fig. 4a). The expressions of norB, norC, norD, and norQ genes of AOB were up-regulated by 2.5, 16.2, 0.7, and 0.2 folds, respectively (Fig. 4c and Supplementary Table 9). These results provide molecular evidence for the observed stimulation of N2O emission under acidified conditions. Although alternative enzymes might have also contributed to nitrifying bacterial N2O emissions, such as cytochrome c554 (encoded by cycA)57, cytochrome c’-β (encoded by cytS)58, and cytochrome P46036, transcripts of these proteins were not observed in this study. In contrast to the dramatically up-regulated expression of nitric oxide reductase genes, transcripts of nitrite reductase (NO-forming, nirK) of nitrifying bacteria were down-regulated by 43% in the acidified treatments compared with the ambient control (P < 0.05) (Fig. 4c and Supplementary Table 9), implying that NO2− reduction by nitrifying bacteria might have been inhibited by acidification. Therefore, the enhanced emission of N2O under acidified conditions was not sourced from the denitrification pathway of bacterial nitrifiers. Although it was suggested that the abiotic hybrid reaction was the main source for archaeal N2O yield32,37,38, presumptive enzymes including copper hydroxylamine oxidoreductase (Cu-HAO) and putative nitroxyl oxidoreductase were speculated to be involved in the N2O production of AOA59. However, transcripts of these presumptive proteins were not observed (Fig. 5a). In addition, archaeal copper nitrite reductase (nirK) may function as bacteria-like HAO to oxidize NH2OH to N2O, or may be involved in the N2O production of AOA via nitrifier denitrification59. Nevertheless, the transcriptional response of archaeal nirK gene may not be the main cause of the increased emission of N2O under acidification, as the expression of archaeal nirK was slightly down-regulated (Fig. 5c). On the contrary, transcripts of the presumptive archaeal nitric oxide reductase norQ gene were up-regulated by 0.2 folds under acidified conditions (Fig. 5c). Whereas, due to the limited understanding of AOA-driven N2O production pathways, the molecular mechanism of AOA-mediated elevation of N2O emission under acidification needs to be further elucidated.

The intracellular pH of cyanobacterium was reported to decrease along with water pH under acidified conditions53. If the case happens for nitrifiers, they may need to generate more proton motive force (PMF), which is required for adenosine triphosphate (ATP) synthesis60. However, the gene transcripts involved in the proton-translocating membrane-bound enzymes of the enriched nitrifying bacteria were down-regulated in the acidified treatments (Log2FC < −1) (Fig. 4a). Nitrifying bacterial transcripts of NADH-quinone oxidoreductase gene (nuo) of complex I, ubiquinol-cytochrome c reductase gene (pet) of complex III, and cytochrome c oxidase gene of complex IV were down-regulated by 60%, 57%, and 61%, respectively, under acidified conditions (Fig. 4c). Thus, based on these data, we cannot distinguish whether the cytoplasmic pH of nitrifying bacteria was reduced under the imposed degree of acidification. Nevertheless, an important insight was obtained when probing into the transcripts of genes encoding the energy-yielding adenosine triphosphatases (ATPases). Bacterial ATPase family comprises membrane-bound protein complexes responsible for either ATP synthesis using a cross-membrane PMF, or establishing PMF using the energy released from ATP hydrolysis61,62. Based on the metatranscriptomic data, transcripts of genes encoding the bacterial V-type ATPases, working as ATP-dependent proton pumps, were up-regulated (up to 11.5-fold increase) under acidified condition (Fig. 4c). This result suggests an increasing need of nitrifying bacteria for pumping cytoplasmic protons to maintain pH homeostasis and the H+ gradient under a reduction of ~0.3 pH units. Similarly, transcripts of archaeal nuo gene of complex I, cytochrome c oxidase cox gene of complex IV, and archaeal V-type ATPase atp genes were significantly up-regulated (Log2FC > 0.5; Fig. 5a), showing that the intracellular pH of the AOA may have also decreased with water pH.

Under acidified conditions, an increasing energy demand for translocation of substrates across membranes might occur, because gene transcripts encoding the corresponding ATP-binding cassette transporters were up-regulated (with a maximum of 11-fold upregulation, Figs. 4c and 5c). These results suggest that more of the energy derived from NH3 or NO2− oxidation may have been allocated to cope with acidification stress, such as to maintain cytosolic pH homeostasis and substrate transports. However, the total production of ATP was probably reduced, as nitrification rates were significantly inhibited by acidification. In addition, transcripts of genes encoding bacterial F-type ATPases, which function as PMF-driven ATP synthases, were down-regulated by acidification (with an average reduction of 63%, Fig. 4c). Nevertheless, the expression of genes associated with the carbon-concentrating mechanism that saturates the carboxylating enzyme, ribulose bisphosphate carboxylase oxidase (Rubisco), was down-regulated under acidified conditions (Log2FC < −1; Fig. 4a), suggesting a reduced energetic requirement for CO2 enrichment63 (Supplementary Text 2). This regulating mechanism may explain the observed “CO2-fertilization” effect under high pCO2 but under ambient pH level (800 μatm/pH 8.1) (Fig. 3a), as more energy was probably saved and reallocated to other cellular processes. However, the saved energy due to elevated carbon source seemed to be minor when compared with the disturbances caused by acidification, as significantly negative effects were observed under acidified conditions (800 μatm/pH 7.8) (Fig. 3a). Collectively, these results suggest that acidification may lead to lower production of ATP and reallocation of this energy to support cell maintenance rather than to fuel chemoautotrophic growth. Indeed, the growth rate of the dominant nitrifying bacteria was reduced at acidified treatments (reduction of ~25% and 27% for Nitrosomonas and Nitrospira, respectively) (Supplementary Fig. 16a,b). Further evidence was observed at the transcriptional level, as gene transcripts involved in CO2 fixation [Calvin cycle for Nitrosomonas and reductive tricarboxylic acid cycle for Nitrospira] were ubiquitously down-regulated (Supplementary Text 3, Fig. 4b,c)25,27. However, gene transcripts involved in 3-hydroxypropionate/4-hydroxybutyrate carbon-fixation pathway of AOA, which is more energetically favorable24,26, were generally up-regulated under acidified conditions (Fig. 5b,c). Consistent with these results, relatively higher AOA abundance was detected in the acidified treatments (P < 0.05) (Supplementary Fig. 16c), due mainly to the chemoautotrophic growth of Nitrosopumilus (the dominant AOA genus) whose relative abundance also increased under acidified conditions (P < 0.05) (Fig. 6a). Thus, aquatic acidification might change the community of ammonia oxidizers towards an increasing ratio of AOA over AOB. This variation might also be caused by higher NH3 affinity of AOA22,64 and the reduced NH3 availability under acidified conditions25. However, ref. 44 reported that AOA assemblage composition was not sensitive to ocean acidification, possibly because the incubation period (less than one week) in their study was not long enough to cause significant turnover in the assemblage.

a Relative abundance of nitrifying microbes based on the 16S rRNA gene sequencing with universal primers capable of detecting both bacteria and archaea within the same sequencing libraries. The inserted plot shows the relative abundance of ammonia-oxidizing archaea (Nitrosopumilus and Nitrososphaera). Single asterisk denotes significant difference at P < 0.05, while double asterisk denotes significant difference at P < 0.01. Error bar represents SD (n = 3 biologically independent samples), and dots are corresponding data points of the replicates. b The proportion of key nitrifying gene transcripts of comammox based on metatranscriptome sequencing data. Horizontal lines in the box charts indicate the medium, stars represent the mean. The boxes give the 25th and 75th percentiles, the whiskers show range from 5th to 95th percentiles, and the curves show the distribution of the values. Single asterisk denotes significant difference at P < 0.05 (n = 6 genes). Significant differences were determined via one-way analysis of variance (ANOVA). Source data are provided as a source data file.

Furthermore, the communities of nitrifying bacteria were affected by acidification (Fig. 6a). The relative abundance of Nitrosomonas (the dominant AOB) decreased in the acidified treatments (P < 0.05), while that of Nitrospira [the dominant nitrite-oxidizing bacteria (NOB), including comammox] increased (P < 0.05). This overall increased ratio of Nitrospira over known ammonia oxidizers indicates an increasing proportion of comammox under acidified conditions. Indeed, the comammox amoA gene abundance was higher in the acidified treatments (P < 0.05) (Supplementary Fig. 16d). In addition, based on metatranscriptome data, higher comammox gene transcripts were detected in the acidified treatments (0.78–11.59% of the total obtained gene transcripts involved in the stepwise oxidation of NH3 to NO3−) compared with the ambient control (only 0.03–0.89%, P < 0.05) (Fig. 6b and Supplementary Fig. 17). Interestingly, the increase was higher for gene transcripts involved in the oxidation of NH3 to NO2− (amo and hao) than those involved in NO2− oxidation (nxr) (Fig. 6b), suggesting the possibility that part of the comammox may only serve as partial, rather than complete, ammonia oxidizers under acidified conditions. However, further investigations are still required to test this hypothesis. These data demonstrate that aquatic acidification has profound impact on nitrifying communities and their physiological metabolism in estuarine and coastal ecosystems.

In conclusion, our findings suggest that the ongoing aquatic acidification due to the synergistic effects of human activity-induced eutrophication and elevated atmospheric CO2 could disrupt a vital link of nitrogen cycle and increase the production of the powerful greenhouse gas N2O in estuarine and coastal waters. Contrary to our expectation, elevated pCO2 did not exhibit “CO2-fertilization” effect on chemoautotrophic nitrifiers under acidified conditions. Nitrifiers respond significantly to acidification at the transcriptional level, and greatly up-regulate gene expressions associated with intracellular pH homeostasis to cope with acidification stress. These results provide important insights about the underlying mechanism of acidification affecting nitrification and associated N2O emission, and we propose that further acidification in estuarine and coastal waters may alter nitrogen cycle and accelerate global warming by stimulating N2O emission.

The Yangtze River is the largest river in the Euro-Asian continent by virtue of water discharge and is also the third longest river in the world. The Yangtze River basin is characterized by rapid economic development and high population density. Due to the intensive human activities, significant amount of reactive N has been discharged into the Yangtze Estuary and adjacent coastal area, leading to serious N pollution and rapid water acidification65. Therefore, the Yangtze Estuary was selected as the study area to investigate the responses of nitrification rate and associated N2O emission to aquatic acidification. To achieve this goal, six representative sampling sites (Yz1 to Yz6) were selected along the estuarine gradient from the estuary mouth to its adjacent coastal area.

In this study, near-bottom waters, which typically exhibit more active nitrification and more severe acidification65,66, were collected from these sites during the March cruise of National Natural Science Foundation of China in 2020, using Niskin-X bottles mounted on a conductivity-temperature-depth (CTD) profiler (Sea-Bird 911 plus) (Fig. 1). Water depth, salinity, pH and DO were recorded with CTD profiler, pH meter (Thermo Orion 3-Star) and Winkler’s method. Part of the water from each site was preserved in dark at 4 °C for subsequent acidification experiments. Meanwhile, known amounts of subsamples were immediately filtered with 0.22 μm pore-size sterile filters (Waterman), and the filtrates were preserved for nutrient analyses while the membranes were carefully preserved under −20 °C for later DNA extraction (Supplementary Methods). An extra amount of subsample from site Yz3 was also preserved at 4 °C in dark for later long-term manipulation experiments.

The acidification experiments were conducted in a series of continuous-flow manipulation systems (constructed based on bioreactors, Infors, Switzerland, with 4.0 L water samples and 1.0 L headspace) by continuously and gently bubbling water samples with humidified and 0.22 μm-filtered synthetic air (79% N2 and 21% O2):CO2 mixtures (20 mL min−1, precisely controlled by mass flow meter). The inlet air:CO2 bubbles were gently dispersed in the water body by two stirrers which were installed at the bottom and above the inlet airflow (~30 rpm), to make the water system homogeneous and to equilibrate the liquid and gas phases. After equilibrium, headspace gas was collected from the reactors using gas-tight syringes for analyses of natural isotopic signatures of N2O to reveal the N2O production pathways (Supplementary Methods). Subsequently, nitrification rates were determined by addition of 15NH4Cl (>98 atom percent 15N, lower than 20% of ambient concentration) as a tracer and the accumulation of 15N in the NOx− (NO3− + NO2−) pools. During rate measurements, water pH was maintained stable via 0.2 M NaOH solution through the reactor’s acid–base automatic regulator, as protons (H+) can be released during the process of NH3 oxidation. Meanwhile, DO concentration was maintained saturated and pCO2 was maintained at the targeted level via a 100 mL h−1 gas flow, and the outflow gas was collected with gas sampling bags (Teflon®FEP, DuPont). Temperature was maintained at room temperature (25 °C) by an automatic heating plate and cold circulating water bath. During the incubation, pH, DO, and temperature were recorded in real time through the equipped pH sensor (Hamilton, Switzerland), DO sensor (Hamilton, Switzerland), and temperature sensor (Infors, Switzerland), respectively. The incubations were conducted in dark by covering the reactor tanks with opaque paper. Liquid samples (30 mL) were collected at the beginning and end of the 24 h incubation, with gas-tight syringes through a reserved sampling tube (clamped tightly after sampling) and filtered immediately (0.22 μm, Waterman). Part of the filtered water was used for measurements of NO3− and NO2−, while the other part was prepared for 15NOx− analysis using the “denitrifier method”67. In addition, another 30 mL water samples were collected for measurements of DIC, alkalinity, and pCO2. Meanwhile, gas samples were extracted using gas-tight syringes for CO2, N2O, and N2O isotope measurements. Before utilization, the reagent solutions were filter-sterilized (0.22 μm, Waterman), and the reactor tank and related materials were heat-sterilized (121 °C and 15 psi for 20 min) (the same below). All the experiments were conducted in triplicate. The nitrification rates were calculated using the following equation68:

where Rnitrification is the nitrification rate (nmol L−1 h−1), \({R}_{t0}{{{NO}}_{X}}^{-}\)and \({R}_{t}{{{NO}}_{X}}^{-}\) are the ratios of 15N in the \({{{NO}}_{X}}^{-}\) pool measured at the initial (t0) and final (t) time of the incubation, respectively. \({\left[{{{NO}}_{X}}^{-}\right]}_{t0}\) and \({\left[{{{NO}}_{X}}^{-}\right]}_{t}\) are the concentrations of \({{{NO}}_{X}}^{-}\) at the initial (t0) and final (t) time of the incubation, respectively. \({\left[{\!\,}^{14}{{{NH}}_{4}}^{+}\right]}\) and \([{\,\!}^{15}{{{NH}}_{4}}^{+}]\) represent the ambient \({{{NH}}_{4}}^{+}\) concentration and the final \({\,\!}^{15}{{{NH}}_{4}}^{+}\) concentration after the addition of the stable isotope tracer, respectively.

N2O production rates were calculated based on the increase in mass 45 and 46 N2O (\({\,\!}^{45}N_{2}\) and \({\,\!}^{46}N_{2}\)) during the experiments69:

where \({R}_{{N}_{2}O}\) is the N2O production rate (pmol N2O-N L−1 h−1). F is the fraction of 15N in the substrate (\({{{NH}}_{4}}^{+}\)) pool, as described above. \({{\,\!}^{45}N_{2}}O_{t0}\) and \({{\,\!}^{45}N_{2}}O_{t}\) indicate the amount of \({\,\!}^{45}N_{2}O\) at the initial (t0) and final (t) time of the incubation, respectively. \({{\,\!}^{46}N_{2}}O_{t0}\) and \({{\,\!}^{46}N_{2}}O_{t}\) are the amount of \({\,\!}^{46}N_{2}O\) at the initial (t0) and final (t) time of the incubation, respectively. V represents the volume of water sample (L).

To distinguish potential effects of increasing pCO2 and decreasing pH on nitrification rates and associated N2O production under acidification, a series of continuous-airflow manipulation systems were constructed similarly as in the acidification experiments. Briefly, four groups of simulation systems were constructed: (a) 400 μatm/pH 8.1 (the ambient control group), (b) 400 μatm/pH 7.8 (reduction of pH only, maintaining pCO2 at the ambient level), (c) 800 μatm/pH 7.8 (the acidification group), (d) 800 μatm/pH 8.1 (increase of pCO2 only, maintaining pH at the ambient level) (Supplementary Fig. 7). 4 L of the collected water samples from site Yz3 was added per reactor, and 15NH4Cl (>98 atom percent 15N) was added to lower than 20% of ambient NH4+ concentrations. The carbonate chemistry was manipulated by steadily bubbling the water samples with 0.22 μm-filtered CO2 adjusted air (400 μatm for groups a and b, 800 μatm for groups c and d) while adjusting pH (7.8 for groups b and c, 8.1 for groups a and d) with sterile acid (0.1 M HCl) or base (0.2 M NaOH) solution via the reactor’s acid-base automatic regulator. Other reaction conditions (temperature, DO, gas flow rate, stirring speed, and dark condition) were the same as in the acidification experiments. Nitrification rates and N2O production rates were measured as described above during 24 h of incubation after equilibrium. All the experiments were conducted in triplicate.

Another two groups of manipulation systems were set up similarly with water samples from site Yz3 for long-term acidification experiments: (a) 400 μatm/pH 8.1 (control group) and (b) 800 μatm/pH 7.8 (acidification group). pCO2 and pH in the water body were manipulated by continuous bubbling with humidified and 0.22 μm-filtered ambient air (400 μatm, group a) or CO2-enriched air (800 μatm, group b), with pH stabilized at 7.8 or 8.1, respectively. During the long-term acidification experiments, NH4+ was supplemented and maintained at around ambient concentrations (below 10 μM) in the reactors (Supplementary Fig. 10). After about half of the NH4+ was consumed, filter-sterilized site water samples with proper NH4+ concentrations were used as culture medium and supplied at a flow rate of 1 L day−1 to all reactors. Sterilized NaOH solution of 0.2 M was used to neutralize the released H+ in the process of nitrification. The DO was maintained saturated and the temperature was maintained at 25 °C as described above. During the incubation, liquid samples (10 ml) were collected from the reactors every day and immediately filtered (0.22 μm, Waterman) for measurements of NH4+, NO3−, and NO2−. 2 ml of the headspace gas was also collected every day using gas-tight syringes for analyses of N2O and CO2. Known amount of sample was collected every several days and pelleted by centrifugation (20,000 g, 5 min). Pellets were immediately used for DNA extraction, and subsequent pyrosequencing and qPCR assays (Supplementary Methods, Supplementary Table 10). Subsamples were also collected every week for measurements of nitrification and N2O production rates. At the end of one-month incubation, samples were harvested, pelleted (20,000 g for 5 min, under 2 °C), and cryopreserved immediately in liquid nitrogen for subsequent metatranscriptomic analyses.

For AOA enrichment, a continuous-flow nitrifying bioreactor (Infors, Switzerland) with a working volume of 4.0 L was set up with fresh water samples from site Yz3. Filter-sterilized site water supplemented with NH4+ (~10 μM) and streptomycin (~50 mg/L, to inhibit nitrifying bacteria70,71) was continuously supplied at a flow rate of ~0.5 L day−1. DO concentration was maintained saturated by flushing continuously with air. pH was maintained at 8.1 with 0.2 M KHCO3 solution through the reactor’s acid-base automatic regulator. The incubations were conducted in dark and the temperature was maintained at 25 °C as described above. During the incubation, liquid samples were collected from the reactor and filtered immediately using 0.22 μm pore size filters (Waterman) for the measurement of NH4+, NO3−, and NO2−. In addition, samples were collected (20,000 g, 5 min) for DNA extraction and subsequent pyrosequencing to monitor the enrichment of AOA (Supplementary Methods). After 50 days of incubation, AOA enrichment culture was harvested to measure nitrification and N2O emission rates.

Liquid samples collected from the reactors were centrifuged (6,000 g, 5 min) to harvest the nitrifying-enriched biomass27. Then, the precipitated biomass at the bottom of the centrifuge tube was washed three times using filter-sterilized site water and re-suspended in it before rate measurements. Subsequently, 100 μl of this suspension was transferred into gas-tight glass vials (120 ml) in which 10 ml of fresh filter-sterilized site water with NH4+ (~10 μM final concentration) was sufficiently bubbled with CO2 adjusted air (400 μatm or 800 μatm). During the incubation, pH was maintained at the targeted level via addition of 3-(N-morpholino) propanesulfonic acid (MOPS, pH adjusted to 8.1 or 7.8, 20 mM final concentration)23. The vials were incubated in dark at 25 °C on an orbital shaker (30 rpm). At each sampling interval (0, 5, 8, 12, and 24 h), three replicates were sacrificed and 2 ml of the headspace gas was extracted for CO2 and N2O analyses. Targeted pH and DO were confirmed using a Mettler-Toledo pH Meter and an OXY Meter S/N 4164 with an oxygen needle sensor (Unisense), respectively. Meanwhile, 5 ml of suspension in each vial was immediately filtered (0.22 μm, Waterman) for NOx− measurements. Nitrification rates were estimated on the basis of the linear changes in NOx− concentrations with time23. Inhibition of nitrification activity was expressed as the percentage reduction of nitrification rate in the acidified treatments compared to the ambient control. Effect of acidification on N2O emission was determined based on the percentage change of N2O concentration (in the headspace of the glass vials) in the acidified treatments relative to the ambient control23.

Total RNA was extracted from triplicate ambient controls (400 μatm/pH 8.1) and acidified treatments (800 μatm/pH 7.8) using EZNA® Soil RNA kit (Omega Bio-tek, Norcross, GA, USA) at the end of incubation. Residual genomic DNA was removed with the Turbo DNA-free kit (Ambion) and further verified through PCR using primers 515F (5′-GTGCCAGCMGCCGCGGTAA-3′) and 909R (5′-CCCCGYCAATTCMTTT RAGT-3′) to rule out DNA contamination23. The purity, integrity, and concentration of the extracted RNA were measured using Agilent2100 (Agilent) and Nanodrop2000 (Thermo). Ribosomal RNA (rRNA) was then removed via Ribo-Zero rRNA Removal Kit (Epicentre) to acquire qualified mRNA, which was used for qPCR analyses (Supplementary Methods) and metatranscriptome sequencing.

Metatranscriptomic cDNA libraries were constructed with the TruSeq RNA Sample Prep Kit (Illumina) and sequenced on an Illumina HiSeq4000 platform after triplicate mRNA samples from the ambient controls and the acidified treatments were pooled individually72. The quality of raw reads was checked via FastQC and trimmed by SeqPrep (https://github.com/jstjohn/SeqPrep). Subsequently, raw reads were quality-filtered using Sickle (https://github.com/najoshi/sickle), and sequences with ambiguous (N) bases, low quality (below 20), and lengths less than 50 base pairs (bp) were discarded. SortMeRNA (http://bioinfo.lifl.fr/RNA/sortmerna/) was further used to screen and remove rRNA reads. The resulting high-quality mRNA clean reads were assembled using Trinity de novo assembly pipeline (contigs less than 200 bp were removed)73. MetaGeneMark (http://exon.gatech.edu/meta_gmhmmp.cgi) was used to predict open reading frames (ORFs), and those longer than 100 bp were translated into amino acid sequences. Non-redundant contigs (95% identity; 90% coverage) were obtained via CD-HIT software (http://www.bioinformatics.org/cd-hit/). Expression levels of the transcripts were calculated via kallisto (https://pachterlab.github.io/kallisto/) and were reported as TPM (Transcripts Per Kilobase Million). Fold change (FC) in relative gene expression was calculated by comparing the acidified treatments to the ambient control. Taxonomic affiliations of the transcripts were assigned via binning to the best hit in the NR database (BLASTP, e-value ≤ 10−5). Potential functions were assigned based on the best homology to proteins within the KEGG (Kyoto Encyclopedia of Genes and Genomes) database (BLASTP, e-value ≤ 10−5). Maximum-likelihood tree was constructed with IQ-TREE74 with 1000 ultrafast bootstraps and then visualized and annotated by iTOL (interactive tree of life)75.

Concentrations of CO2 and N2O were monitored by gas chromatography (GC-2014, Shimadzu, Kyoto, Japan). N2O isotope ratios (m/z = 44, 45, 46) were analyzed on isotope ratio mass spectrometry (IRMS, Delta V Advantage, Thermo Fisher Scientific, Bremen, Germany). Natural N2O isotopic signatures (δ15Nbulk, δ15Nα, and δ18O) for revealing N2O production pathways were analyzed on IRMS (Delta V Plus, Thermo Fisher Scientific, Bremen, Germany). Concentrations of NH4+, NO3− and NO2− were measured colorimetrically using a continuous-flow nutrient analyzer (Skalar SANplus, Skalar Analytical BV, Breda, The Netherlands) with detection limits of 0.3 μM for NH4+-N and 0.05 μM for NO2−-N and NO3−-N. DIC concentration was analyzed by acidification and subsequent quantification of released CO2 (Carbon coulometer, UIC-INC, America). Alkalinity and pCO2 were calculated via the CO2SYS program76, on the basis of pH and DIC measurements using the carbonic acid dissociation constants of ref. 77 that were refit in different functional forms by ref. 78.

Statistical analyses were performed using SPSS version 19.0 for Windows (SPSS Inc., Chicago, I L. USA). Significant differences among differently treated groups were identified via one-way analysis of variance (ANOVA) followed by Tukey’s honestly test. Nonlinear fitted curves (polynomial fit) were constructed using Origin 2022b to explore the responses of nitrification and associated N2O production rates to different levels of acidification. Results were considered significant when P < 0.05.

Further information on research design is available in the Nature Portfolio Reporting Summary linked to this article.

All sequence data and sample information are available at National Center for Biotechnology Information (NCBI) Sequence Read Archive (SRA) database under BioProject accession numbers PRJNA876082. All data needed to evaluate the conclusions in the paper are present in the paper and/or the Supplementary Materials. Source data are provided with this paper.

Hong, C. P. et al. Global and regional drivers of land-use emissions in 1961–2017. Nature 589, 554–561 (2021).

Article ADS CAS PubMed Google Scholar

World Meteorological Organization, State of the Global Climate 2021: WMO Provisional report. WMO-No.1290 (2022).

IPCC, Global carbon and other biogeochemical cycles and feedbacks, in climate change 2021: the physical science basis. Contribution of Working Group I to the Sixth Assessment Report of the Intergovernmental Panel on Climate Change, Masson-Delmotte, V. et al. Eds. (Cambridge Univ. Press, 2021).

Gruber, N. et al. The oceanic sink for anthropogenic CO2 from 1994 to 2007. Science 363, 1193–1199 (2019).

Article ADS CAS PubMed Google Scholar

Caldeira, K. & Wickett, M. E. Anthropogenic carbon and ocean pH. Nature 425, 365 (2003).

Article ADS CAS PubMed Google Scholar

Botté, E. S. et al. Changes in the metabolic potential of the sponge microbiome under ocean acidification. Nat. Commun. 10, 4134 (2019).

Article ADS PubMed PubMed Central Google Scholar

Hoegh-Guldberg, O. et al. Coral reefs under rapid climate change and ocean acidification. Science 318, 1737–1742 (2007).

Article ADS CAS PubMed Google Scholar

Cavicchioli, R. et al. Scientists’ warning to humanity: microorganisms and climate change. Nat. Rev. Microbiol. 17, 569–586 (2019).

Article CAS PubMed PubMed Central Google Scholar

Bianchi, T. S. & Allison, M. A. Large-river delta-front estuaries as natural “recorders” of global environmental change. Proc. Natl Acad. Sci. U.S.A. 106, 8085–8092 (2009).

Article ADS CAS PubMed PubMed Central Google Scholar

Doney, S. C. The growing human footprint on coastal and open-ocean biogeochemistry. Science 328, 1512–1516 (2010).

Article ADS CAS PubMed Google Scholar

Scanes, E., Scanes, P. R. & Ross, P. M. Climate change rapidly warms and acidifies Australian estuaries. Nat. Commun. 11, 1803 (2020).

Article ADS CAS PubMed PubMed Central Google Scholar

Cai, W. J. et al. Natural and anthropogenic drivers of acidification in large estuaries. Ann. Rev. Mar. Sci. 13, 23–55 (2021).

Article ADS PubMed Google Scholar

Laurent, A. et al. Eutrophication-induced acidification of coastal waters in the northern Gulf of Mexico: insights into origin and processes from a coupled physical-biogeochemical model. Geophys. Res. Lett. 44, 946–956 (2017).

Article ADS CAS Google Scholar

Waldbusser, G. G. & Salisbury, J. E. Ocean acidification in the coastal zone from an organism’s perspective: multiple system parameters, frequency domains, and habitats. Ann. Rev. Mar. Sci. 6, 221–247 (2014).

Article PubMed Google Scholar

Teixidó, N. et al. Functional biodiversity loss along natural CO2 gradients. Nat. Commun. 9, 5149 (2018).

Article ADS PubMed PubMed Central Google Scholar

Su, X. X. et al. Stimulation of N2O emission via bacterial denitrification driven by acidification in estuarine sediments. Glob. Change Biol. 27, 5564–5579 (2021).

Article CAS Google Scholar

Cyronak, T., Schulz, K. G., Santos, I. R. & Eyre, B. D. Enhanced acidification of global coral reefs driven by regional biogeochemical feedbacks. Geophys. Res. Lett. 41, 5538–5546 (2014).

Article ADS CAS Google Scholar

Cai, W. J. et al. Redox reactions and weak buffering capacity lead to acidification in the Chesapeake Bay. Nat. Commun. 8, 369 (2017).

Article ADS PubMed PubMed Central Google Scholar

Su, J. Z. et al. Chesapeake Bay acidification buffered by spatially decoupled carbonate mineral cycling. Nat. Geosci. 13, 441–447 (2020).

Article ADS CAS Google Scholar

Bednaršek, N. et al. Severe biological effects under present-day estuarine acidification in the seasonally variable Salish Sea. Sci. Total Environ. 765, 142689 (2021).

Article ADS PubMed Google Scholar

Wu, L. et al. Effects of aquatic acidification on microbially mediated nitrogen removal in estuarine and coastal environments. Environ. Sci. Technol. 56, 5939–5949 (2022).

Article ADS CAS PubMed Google Scholar

Martens-Habbena, W., Berube, P. M., Urakawa, H., de la Torre, J. R. & Stahl, D. A. Ammonia oxidation kinetics determine niche separation of nitrifying Archaea and Bacteria. Nature 461, 976–979 (2009).

Article ADS CAS PubMed Google Scholar

Zheng, Y. L. et al. Effects of silver nanoparticles on nitrification and associated nitrous oxide production in aquatic environments. Sci. Adv. 3, e1603229 (2017).

Article ADS PubMed PubMed Central Google Scholar

Berg, I. A., Kockelkorn, D., Buckel, W. & Fuchs, G. A. 3-hydroxypropionate/4-hydroxybutyrate autotrophic carbon dioxide assimilation pathway in Archaea. Science 318, 1782–1786 (2007).

Article ADS CAS PubMed Google Scholar

Ward, B. B. Nitrification in marine systems.in Nitrogen in the Marine Environment, pp. 199–261 (Elsevier, Amsterdam, 2008).

Walker, C. B. et al. Nitrosopumilus maritimus genome reveals unique mechanisms for nitrification and autotrophy in globally distributed marine crenarchaea. Proc. Nat. Acad. Sci. U. S. A. 107, 8818–8823 (2010).

Article ADS CAS Google Scholar

Daims, H. et al. Complete nitrification by Nitrospira bacteria. Nature 528, 504–509 (2015).

Article ADS CAS PubMed PubMed Central Google Scholar

Beman, J. M. et al. Global declines in oceanic nitrification rates as a consequence of ocean acidification. Proc. Natl Acad. Sci. U.S.A. 108, 208–213 (2011).

Article ADS CAS PubMed Google Scholar

Zeebe, R. E. & Wolf-Gladrow, D. CO2 in seawater: equilibrium, kinetics, isotopes. in Elsevier Oceanography Series 65 pp. 269–270 (Elsevier, Amsterdam, 2001).

Ishii, S. et al. Identification of key nitrous oxide production pathways in aerobic partial nitrifying granules. Environ. Microbiol. 16, 3168–3180 (2014).

Article CAS PubMed Google Scholar

Santoro, A. E. The do-it-all nitrifier. Science 351, 342–343 (2016).

Article ADS CAS PubMed Google Scholar

Santoro, A. E., Buchwald, C., McIlvin, M. R. & Casciotti, K. L. Isotopic signature of N2O produced by marine ammonia-oxidizing archaea. Science 333, 1282–1285 (2011).

Article ADS CAS PubMed Google Scholar

Hutchins, D. A. & Capone, D. G. The marine nitrogen cycle: new developments and global change. Nat. Rev. Microb. 20, 401–414 (2022).

Article CAS Google Scholar

Ravishankara, A. R., Daniel, J. S. & Portmann, R. W. Nitrous oxide (N2O): The dominant ozone depleting substance emitted in the 21st century. Science 326, 123–125 (2009).

Article ADS CAS PubMed Google Scholar

Kozlowski, J. A., Price, J. & Stein, L. Y. Revision of N2O-producing pathways in the ammonia-oxidizing bacterium Nitrosomonas europaea ATCC 19718. Appl. Environ. Microbiol. 80, 4930–4935 (2014).

Article ADS PubMed PubMed Central Google Scholar

Caranto, J. D., Vilbert, A. C. & Lancaster, K. M. Nitrosomonas europaea cytochrome P460 is a direct link between nitrification and nitrous oxide emission. Proc. Natl Acad. Sci. U. S. A. 113, 14704–14709 (2016).

Article ADS CAS PubMed PubMed Central Google Scholar

Stieglmeier, M. et al. Aerobic nitrous oxide production through N-nitrosating hybrid formation in ammonia-oxidizing archaea. ISME J. 8, 1135–1146 (2014).

Article CAS PubMed PubMed Central Google Scholar

Kozlowski, J. A., Stieglmeier, M., Schleper, C., Klotz, M. G. & Stein, L. Y. Pathways and key intermediates required for obligate aerobic ammonia-dependent chemolithotrophy in bacteria and Thaumarchaeota. ISME J. 10, 1836–1845 (2016).

Article CAS PubMed PubMed Central Google Scholar

Hink, L., Nicol, G. W. & Prosser, J. I. Archaea produce lower yields of N2O than bacteria during aerobic ammonia oxidation in soil. Environ. Microbiol. 19, 4829–4837 (2017).

Article CAS PubMed Google Scholar

Kits, K. D. et al. Low yield and abiotic origin of N2O formed by the complete nitrifier Nitrospira inopinata. Nat. Commun. 10, 1836 (2019).

Article ADS PubMed PubMed Central Google Scholar

Han, P. et al. N2O and NOy production by the comammox bacterium Nitrospira inopinata in comparison with canonical ammonia oxidizers. Water Res. 190, 116728 (2021).

Article CAS PubMed Google Scholar

Breider, F. et al. Response of N2O production rate to ocean acidification in the western North Pacific. Nat. Clim. Change 9, 954–958 (2019).

Article ADS CAS Google Scholar

Fulweiler, R. W., Emery, H. E., Heiss, E. M. & Berounsky, M. V. Assessing the role of pH in determining water column nitrification rates in a coastal system. Estuar. Coast 34, 1095–1102 (2011).

Article CAS Google Scholar

Rees, A. P., Brown, I. J., Jayakumar, A. & Ward, B. B. The inhibition of N2O production by ocean acidification in cold temperate and polar waters. Deep-Sea Res. Pt. II 127, 93–101 (2016).

Article CAS Google Scholar

Frame, C. H., Lau, E., Nolan, E. J. IV, Goepfert, T. J. & Lehmann, M. F. Acidification enhances hybrid N2O production associated with aquatic ammonia-oxidizing microorganisms. Front. Microbiol. 7, 2104 (2017).

Article PubMed PubMed Central Google Scholar

Gattuso, J. P. Approaches and tools to manipulate the carbonate chemistry. in: Guide to Best Practices for Ocean Acidification Research and Data Reporting, pp. 41–51 (European Union, Luxembourg, 2010).

Schulz, K. G., e Ramos, J. B., Zeebe, R. E. & Riebesell, U. CO2 perturbation experiments: similarities and differences between dissolved inorganic carbon and total alkalinity manipulations. Biogeosciences 6, 2145–2153 (2009).

Article ADS CAS Google Scholar

Zhu, X., Burger, M., Doane, T. A. & Horwath, W. R. Ammonia oxidation pathways and nitrifier denitrification are significant sources of N2O and NO under low oxygen availability. Proc. Nat. Acad. Sci. U.S.A. 110, 6328–6333 (2013).

Article ADS CAS Google Scholar

Dalsgaard, T. et al. Oxygen at nanomolar levels reversibly suppresses process rates and gene expression in anammox and denitrification in the oxygen minimum zone off northern chile. mBio 5, e01966–14 (2014).

Article CAS PubMed PubMed Central Google Scholar

Murray, R. H., Erler, D. V. & Eyre, B. D. Nitrous oxide fluxes in estuarine environments: response to global change. Glob. Chang. Biol. 21, 3219–3245 (2015).

Article ADS PubMed Google Scholar

Seitzinger, S. P., Kroeze, C. & Styles, R. V. Global distribution of N2O emissions from aquatic systems: natural emissions and authropogenic effects. Chemosphere 2, 267–279 (2000).

CAS Google Scholar

Stocker, T. F. et al. IPCC Climate change 2013: The physical science basis. Contribution of Working Group I to the fifth assessment report of the intergovernmental panel on climate change (Cambridge Univ. Press, 2013).

Hong, H. Z. et al. The complex effects of ocean acidification on the prominent N2-fixing cyanobacterium Trichodesmium. Science 356, 527–531 (2017).

Article ADS CAS PubMed Google Scholar

Hutchins, D. A., Mulholland, M. R. & Fu, F. X. Nutrient cycles and marine microbes in a CO2-enriched ocean. Oceanography 22, 128–145 (2009).

Article Google Scholar

de Nadal, E., Ammerer, G. & Posas, F. Controlling gene expression in response to stress. Nat. Rev. Genet. 12, 833–845 (2011).

Article PubMed Google Scholar

Lawton, T. J., Ham, J., Sun, T. L. & Rosenzweig, A. C. Structural conservation of the B subunit in the ammonia monooxygenase/particulate methane monooxygenase superfamily. Proteins 82, 2263–2267 (2014).

Article CAS PubMed PubMed Central Google Scholar

Upadhyay, A. K., Hooper, A. B. & Hendrich, M. P. NO reductase activity of the tetraheme cytochrome C554 of Nitrosomonas europaea. J. Am. Chem. Soc. 128, 4330–4337 (2006).

Article CAS PubMed PubMed Central Google Scholar

Stein, L. Y. Surveying N2O-producing pathways in bacteria. Methods Enzymol. 486, 131–152 (2011).

Article CAS PubMed Google Scholar

Wu, L. et al. A critical review on nitrous oxide production by ammonia-oxidizing archaea. Environ. Sci. Technol. 54, 9175–9190 (2020).

Article ADS CAS PubMed Google Scholar

Spero, M. A., Aylward, F. O., Currie, C. R. & Donohue, T. J. Phylogenomic analysis and predicted physiological role of the proton-translocating NADH:quinone oxidoreductase (complex I) across bacteria. mBio 6, e00389–15 (2015).

Article PubMed PubMed Central Google Scholar

Zhou, L. & Sazanov, L. A. Structure and conformational plasticity of the intact Thermus thermophilus V/A-type ATPase. Science 365, eaaw9144 (2019).

Article CAS PubMed Google Scholar

Wang, B. Z. et al. Expansion of Thaumarchaeota habitat range is correlated with horizontal transfer of ATPase operons. ISME J. 13, 3067–3079 (2019).

Article CAS PubMed PubMed Central Google Scholar

Badger, M. R. & Bek, E. J. Multiple Rubisco forms in proteobacteria: Their functional significance in relation to CO2 acquisition by the CBB cycle. J. Exp. Bot. 59, 1525–1541 (2008).

Article CAS PubMed Google Scholar

Zhang, Y. et al. Nitrifier adaptation to low energy flux controls inventory of reduced nitrogen in the dark ocean. Proc. Natl Acad. Sci. U.S.A. 117, 4823–4830 (2020).

Article ADS CAS PubMed PubMed Central Google Scholar

Guo, X. H. et al. Seasonal variability and future projection of ocean acidification on the east China sea shelf off the Changjiang Estuary. Front. Mar. Sci. 8, 770034 (2021).

Article ADS Google Scholar

Wang, W. T. et al. Rates of nitrification and nitrate assimilation in the Changjiang River estuary and adjacent waters based on the nitrogen isotope dilution method. Cont. Shelf Res. 163, 35–43 (2018).

Article ADS Google Scholar

Sigman, D. M. et al. A bacterial method for the nitrogen isotopic analysis of nitrate in seawater and freshwater. Anal. Chem. 73, 4145–4153 (2001).

Article CAS PubMed Google Scholar

Lu, Y. H. et al. New insight to niche partitioning and ecological function of ammonia oxidizing archaea in subtropical estuarine ecosystem. Biogeosciences 17, 6017–6032 (2020).

Article ADS CAS Google Scholar

Santoro, A. E. et al. Nitrification and nitrous oxide production in the offshore waters of the eastern tropical South Pacific. Glob. Biogeochem. Cycles 35, e2020GB006716 (2020).

ADS Google Scholar

Qin, W. et al. Marine ammonia-oxidizing archaeal isolates display obligate mixotrophy and wide ecotypic variation. Proc. Nat. Acad. Sci. U.S.A. 111, 12504–12509 (2014).

Article ADS CAS Google Scholar

Rodriguez, J. et al. Nutrient-Limited enrichments of nitrifiers from soil yield consortia of Nitrosocosmicus-affiliated AOA and Nitrospira-affiliated NOB. Front. Microbiol. 12, 671480 (2021).

Article PubMed PubMed Central Google Scholar

Cai, Y. F., Zheng, Y., Bodelier, P. L. E., Conrad, R. & Jia, Z. J. Conventional methanotrophs are responsible for atmospheric methane oxidation in paddy soils. Nat. Commun. 7, 11728 (2016).

Article ADS CAS PubMed PubMed Central Google Scholar

Grabherr, M. G. et al. Full-length transcriptome assembly from RNA-Seq data without a reference genome. Nat. Biotechnol. 29, 644–652 (2011).

Article CAS PubMed PubMed Central Google Scholar

Nguyen, L. T., Schmidt, H. A., von Haeseler, A. & Minh, B. Q. IQ-TREE: a fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies. Mol. Biol. Evol. 32, 268–274 (2015).

Article CAS PubMed Google Scholar

Letunic, I. & Bork, P. Interactive tree of life (iTOL) v4: recent updates and new developments. Nucleic Acids Res. 47, W256–W259 (2019).

Article CAS PubMed PubMed Central Google Scholar

Pierrot, D., Lewis, E. & Wallace, D. CO2SYS (Carbon Dioxide Information Analysis Center, 2006).

Mehrbach, C., Culberson, C. H., Hawley, J. E. & Pytkowicz, R. M. Measurement of the apparent dissociation constants of carbonic acid in seawater at atmospheric pressure. Limnol. Oceanogr. 18, 897–907 (1973).

Article ADS CAS Google Scholar

Dickson, A. G. & Millero, F. J. A comparison of the equilibrium constants for the dissociation of carbonic acid in seawater media. Deep-Sea Res 34, 1733–1743 (1987).

Article ADS CAS Google Scholar

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This work is supported by the National Natural Science Foundation of China (Nos. 41725002 [L.J.H.], 41971105 [Y.L.Z.], 42222605 [Y.L.Z.], 42030411 [L.J.H.], 42249903 [L.J.H.], 41671463 [L.J.H.], and 41730646 [M.L.]), the Chinese National Key Programs for Fundamental Research and Development (No. 2016YFA0600904) [L.J.H.], and Director’s Fund of Key Laboratory of Geographic Information Science (Ministry of Education), East China Normal University (Grant No. KLGIS2022C03) [Y.L.Z.]. Samples were collected during the open research cruises to Yangtze Estuary (NORC2020-03 and NORC2023-302). We thank W. S. Gardner and Silvia E. Newell for discussion and revision on the manuscript.

These authors contributed equally: Jie Zhou, Yanling Zheng.

State Key Laboratory of Estuarine and Coastal Research, Yangtze Delta Estuarine Wetland Ecosystem Observation and Research Station, East China Normal University, Shanghai, 200241, China

Jie Zhou, Yanling Zheng, Lijun Hou, Zhirui An, Feiyang Chen, Bolin Liu, Hongpo Dong, Xia Liang, Xiaofei Li & Dengzhou Gao

School of Geographic Sciences, East China Normal University, Shanghai, 200241, China

Yanling Zheng, Li Wu, Lin Qi, Ping Han, Guoyu Yin, Yi Yang, Ye Li & Min Liu

Key Laboratory of Geographic Information Science (Ministry of Education), East China Normal University, Shanghai, 200241, China

Yanling Zheng, Li Wu, Lin Qi, Ping Han, Guoyu Yin, Yi Yang, Ye Li & Min Liu

Key Laboratory of Spatial-temporal Big Data Analysis and Application of Natural Resources in Megacities, Ministry of Natural Resources, Shanghai, 200241, China

Yanling Zheng, Ping Han, Guoyu Yin, Yi Yang, Ye Li & Min Liu

The University of Texas at Austin Marine Science Institute, Port Aransas, TX, 78373, USA

Zhanfei Liu

Norwegian Institute for Water Research, Thormøhlensgt 53D, 5006, Bergen, Norway

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Y.L.Z., L.J.H., and M.L. conceived the research. J.Z., Y.L.Z., L.J.H., Z.R.A., F.Y.C., B.L.L., L.W., and L.Q. performed the research. J.Z., Y.L.Z., L.J.H., H.P.D., P.H., G.Y.Y., X.L., Y.Y., X.F.L., D.Z.G., Y.L., Z.F.L., R.B., and M.L. analyzed the data. J.Z., Y.L.Z., L.J.H., R.B., and M.L. wrote the paper.

Correspondence to Yanling Zheng, Lijun Hou or Min Liu.

The authors declare no competing interests.

Nature Communications thanks Yong-guan Zhu and the other, anonymous, reviewer(s) for their contribution to the peer review of this work. Peer reviewer reports are available.

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Zhou, J., Zheng, Y., Hou, L. et al. Effects of acidification on nitrification and associated nitrous oxide emission in estuarine and coastal waters. Nat Commun 14, 1380 (2023). https://doi.org/10.1038/s41467-023-37104-9

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Received: 16 September 2022

Accepted: 28 February 2023

Published: 13 March 2023

DOI: https://doi.org/10.1038/s41467-023-37104-9

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